Part:BBa_K277023

3L.3_23.A2.11

3L.3_23.A2.11 is 645 bases long and is cloned into the pGem-T vector.

3L.3_23.A2.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.11 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

reverse_primer YCL057W_tagr2v1 (126..153)

gene YCL057W (-1418..+720) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins

Sequence (the first 645 bases correspond to coordinates 16322..16966 in synthetic chromosome yeast_chr3_3_23):

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 570
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]